瓜类细菌性果斑病菌luxR基因的功能分析

luxR基因作为信号分子受体蛋白的编码基因,在革兰氏阴性菌群体感应(quorum sensing,QS)LuxI/LuxR环路中起重要作用.本研究采用PCR技术从瓜类细菌性果斑病菌(Acidovgorax avenae subsp.citrulli)xjL12中扩增出luxR同源基因,应用同源重组的方法构建了突变株(△luxR),并对其功能进行初步研究.实验结果表明,△luxR与野生型菌株相比产生的群体感应信号分子浓度降低,抗生素耐受水平有所提高,对哈密瓜(Cucuumis melo var.cantalupensis))幼苗的致病能力下降;RT-PCR实验表明,luxR基因的缺失对luxI基因的表达有抑制作用.本研究为今后对以luxR基因为靶标,利用信号干扰技术综合防治瓜类细菌性果斑病提供基础资料.     

LC-MS/MS quantification of bioactive angiotensin I-converting enzyme inhibitory peptides in rye malt sourdoughs

This study quantified antiotensin I-converting enzyme (ACE) inhibitory peptides in rye malt sourdoughs supplemented with gluten proteins and fermented with six strains of Lactobacillus spp. Bioinformatic analysis of prolamins from barley, rye, and wheat demonstrated that the ACE inhibitory peptides LQP, LLP, VPP, and IPP are frequently encrypted in their primary sequence. These tripeptides were quantified by liquid chromatography-tandem mass spectrometry. Tripeptide levels in sourdoughs were generally higher as compared to the chemically acidified controls. Sourdoughs fermented with different strains showed different concentrations of LQP and LLP. These differences corresponded to strain-specific differences in PepO and PepN activities. The highest levels of peptides VPP, IPP, LQP, and LLP, 0.23, 0.71, 1.09, and 0.09 mmol (kg DM)(-1), respectively, were observed in rye malt: gluten sourdoughs fermented with Lactobacillus reuteri TMW 1.106 and added protease. These concentrations were 6-7 times higher as compared to sourdough without fungal protease and exceed the IC(50) by 100-1000-fold.     

Structure characterization and antioxidant activity of a novel polysaccharide isolated from pulp tissues of Litchi chinensis

A novel polysaccharide (LCP50S-2) with antioxidant activity was isolated from Litchi chinensis Sonn. The structure of LCP50S-2 was elucidated on the basis of physicochemical and instrumental analyses, and its average molecular weight was determined by gel permeation chromatography to be 2.19 × 10(2) kDa. The backbone of LCP50S-2 was composed of (1→3)-linked β-L-rhamnopyranosyl residues, (1→4)-linked α-D-xylopyranosyl residues, (1→4)-linked β-D-glucopyranosyl residues, and (1→4)-linked α-D-glucopyranosyl residues which branched at O-6. The two branches consisted of α-L-arabinopyranosyl residues and (1→6)-linked β-D-galactopyranosyl residues terminated with α-L-arabinopyranosyl residues, respectively. In the in vitro antioxidant assay, LCP50S-2 was found to possess DPPH radical-scavenging activity and hydroxyl radical-scavenging activity with IC(50) values of 220 and 266 μg/mL, respectively.     

Acetylation of chicken feathers for thermoplastic applications

Poultry feathers are renewable resources, inexpensive and abundantly available, but have limited applications. Although keratin extracted from feathers has been chemically modified, there are no reports on the chemical modification or development of thermoplastics from poultry feathers. Acetylation is an inexpensive and environmentally friendly approach to make biopolymers thermoplastic. Several biopolymers have been acetylated and used to produce fibers, films, and extrudates. In this research, chicken feathers were acetylated, and the structure and properties of the acetylated feathers were studied. Acetylation conditions such as concentration of chemicals and catalyst and time and temperature of acetylation were optimized. Acetylation of feathers was confirmed using Fourier transform infrared (FTIR) and pyrolysis-gas chromatography-mass spectrometry (P-GC-MS). The acetylated feathers were analyzed using thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) to understand their thermal behavior. Acetylated feathers were thermoplastic and could be compression molded to form transparent films despite the relatively low percentage of acetyl content.     

基于黄河泥沙充填复垦采煤沉陷地覆土材料的优选

黄河泥沙充填复垦采煤沉陷地需要覆盖足够厚度的土壤以保证农作物生长,针对济宁市部分采煤沉陷地可取土量不足问题,该文提出利用当地表土、心土和黄河泥沙组配作为覆土材料研究,以达到增加覆盖土壤厚度和改良当地土壤质地的目的。研究表明,覆土材料有利于玉米生长发育,表土、心土和黄河泥沙组配(质量比)为1:1:1.33~1:1:2的覆土材料玉米苗期生物量显著高于对照土壤;组配为1:1:0.86~1:1:2的覆土材料质地为壤土,是较为理想的土壤类型;组配为1:1:0.86~1:1:2的覆土材料容重为1.38~1.41g/cm3,密度为2.64~2.68g/cm3,较为适宜农作物生长。覆土材料可以显著降低当地土壤p H值和电导率,对农作物不产生盐害作用;其入渗性能提升,增加了土壤储水能力,组配为1:1:0.86~1:1:2的覆土材料入渗能力接近多年耕种农田;其水分特征曲线左移,毛管孔隙度、有效水孔隙度和水分常数基本呈现下降趋势,持水和供水能力下降。根据尽量增加黄河泥沙充填层上覆土材料厚度原则,优选出表土、心土和黄河泥沙组配为1:1:2的覆土材料作为黄河泥沙充填复垦采煤沉陷地的覆土材料,可在大田试验中进行进一步的验证。     

生态景观型灌排系统面源污染防治试验及生态响应

设计由灌排合一生态环沟、生态景观型斗渠和斗沟组成的新型土地整治灌排系统,以上海市临港土地整治项目区为试验场地进行不同灌排系统面源污染防治效果对比试验,最后引入生态服务价值模型分析其产生的生态响应。结果表明:1)生态景观型灌排系统涵盖稻田-萍-鱼-蔬菜种植版互利共生系统和生态环沟-迂回式生化塘-斗沟湿地面源污染防治系统,实现了稻田养鱼和农业现代化经营共赢;同时在土地整治灌排系统稻田排水口处设置迂回式生化塘,为农业面源污染防治提供了一种新思路;2)生态景观型灌排系统通过植物吸收和截留、基质和底泥吸附及细菌和微生物降解等作用,在水稻生长周期6-10月面源污染防治效果均明显高于其他灌排系统,COD、NH4+-N、TN、TP的平均去除率分别为77.57%、78.99%、69.77%、73.74%;3)生态景观型灌排系统带来的(正,负)生态响应中,正响应为4.95万元,是负响应的45倍,气候调节、水源涵养、废物处理、生物多样性保护、土壤保持和提供美学景观等功能均显著增强。     

风幕式静电喷杆喷雾喷头雾化与雾滴沉积性能试验

为了解决传统施药方式药液在植株中下冠层沉积量不足、雾滴粒径分布不均匀等问题,对常规喷杆喷雾扇形喷头设计了一种双平板感应式荷电装置,试验测量了该扇形静电喷头产生的雾滴荷质比、空间上的横向和纵向粒径分布并验证了在风幕和静电作用下雾滴的沉积性能。试验结果表明:荷质比随着静电电压的增大先增大后趋于稳定,随喷雾压力的增大而减小;静电作用能够减小雾滴粒径,并且使雾滴粒径横向分布更加均匀;随着喷头远离测量装置,纵向雾滴粒径逐渐增大;风幕作用能够改善雾滴在冠层中的沉积性能,相同条件下,有风幕时雾滴沉积分布的变异系数为0.645,与无风幕时的0.871降低了25.95%;静电作用能够改善雾滴在冠层中的沉积性能,在静电电压0和6 kV作用下,雾滴沉积分布的变异系数减小了50.2%;荷电条件下,随着喷雾压力的增大,雾滴的沉积分布均匀性反而会减小。该研究可为进一步研究新型喷头荷电装置及风幕式喷杆喷雾机的研发提供参考。     

基于网络集成的弧齿锥齿轮数字化闭环制造技术

为突破弧齿锥齿轮数字化闭环制造过程信息继承性与集成性低的局限,实现弧齿锥齿轮齿面数字化闭环过程的集成运行和齿面制造信息的集成管理,该文提出了一种弧齿锥齿轮的网络化闭环制造模型,建立了网络化闭环制造的运行机制,采用基于TCP/IP(transmission control protocol/internet protocol,传输控制/网络通讯协定)的分布式集成控制模式构建网络化闭环制造模型的通信网络,利用Web服务应用集成方法建立闭环制造过程信息集成机制,并通过设计开发闭环制造集成系统实现网络化闭环制造信息的集成统一管理。应用结果表明:该模型及其实现技术可行实用,实现了齿面制造过程的数字化和集成化运行,使齿轮副的齿面精度和啮合性能都得到明显提高。以该文的准双曲面齿轮副滚动检测试验为例,辐射噪声降低约7%,振动加速度幅值降低约15%。该研究为弧齿锥齿轮的网络化和集成化制造提供参考。     

马唐牧草红壤氮肥的氨挥发、径流和淋溶损失

探讨了我国南方红壤上种植牧草马唐施不同量氮肥,施氮量与土壤氨挥发、径流和1 m深土壤淋溶损失氮量的关系。结果表明,在施用N 90、160、230 kg hm-2尿素处理下,土壤氨挥发损失量分别为N0.67、1.24和5.16 kg hm-2,分别占施氮量的0.74%0、.77%和2.24%,土壤氨挥发损失量(y)与施氮量(x)呈指数递增关系:y=0.156 3e0.014 6x;径流氮素损失量分别为N 1.05、0.88和1.01 kg hm-2,分别占施氮量的1.17%、0.55%和0.44%,径流损失的氮量与施氮量之间无明显相关性;淋溶损失总氮量为2.05、2.86和4.09kg hm-2,分别占施氮量的0.91%、1.02%和1.24%,土壤淋溶损失总氮量(y)与施氮量(x)呈线性递增关系:y=0.012 2x 1.087 7。     

蚓粪对黑麦草吸收污染土壤重金属铜的影响

在长江冲积物形成的高沙土中加入CuSO4.5H2O模拟土壤污染,使Cu污染浓度分别为200、400、600 mg kg-1,并设置加蚓粪(15%)和加原土(15%)处理,二处理各设种植黑麦草(Lolium multiflorum)和不种黑麦草培养试验,研究蚓粪对黑麦草生长及对Cu吸收的影响,以揭示蚓粪在Cu污染土壤植物修复中的作用。结果表明:蚓粪显著增加了黑麦草的地上部和地下部的生物量(p<0.001**)以及根系的长度、表面积、体积和根尖数(p<0.05*),在Cu浓度为200 mg kg-1时促进作用最大;蚓粪还显著提高了黑麦草地上部Cu的浓度及累积量,但显著降低了地下部的Cu浓度(p<0.05*),而对地下部的铜累积量没有影响,显示蚓粪能促进Cu从根系向地上部的运移及在地上部的富集。通过对土壤的pH和Cu的形态特征分析,发现种植黑麦草处理降低了土壤的pH并增加了可交换态铜的含量,而且这种作用在加入蚓粪后更加明显。推测蚓粪主要通过促进黑麦草根系的生长和活性而影响根系周围环境,提高重金属铜的生物有效性进而增加植物对铜的吸收。